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焚膏繼晷
[新知]脂肪細胞要如何保存?
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plasticsuper
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註冊時間: 2005-08-04
文章: 623
來自: 台北

發表 發表於: 星期五 十一月 16, 2007 10:50 am
發表 文章主題: [新知]脂肪細胞要如何保存?
引言回覆

把人体脂肪細胞抽出,再注入其它部位填充,這是很多人覺得理所當然而且很期待的事。但在臨床實做上,卻發現有不少問題待克服,例如抽出的脂肪需要離心以便和其它油、水分離 ,這離心速度會影響脂肪細胞的存活率。分離後的脂肪細胞要泡在什麼樣的溶液才能增加存活?一次該注入多少才能避免缺血壞死和硬化?等等這些變數都還沒有一個定論。因此,不論在研究上或臨床上,各種處理脂肪細胞的方法可謂百花齊放百家爭鳴。

以下這篇文章是探討脂肪和脂肪幹細胞在從身体抽取出後,要在什麼溫度下存放?最久可以存放幾天這二個問題。

引言回覆:

Author Matsumoto, Daisuke M.D.; Shigeura, Tomokuni M.S.; Sato, Katsujiro M.D.; Inoue, Keita M.D.; Suga, Hirotaka M.D.; Kato, Harunosuke M.D.; Aoi, Noriyuki M.D.; Murase, Syoko Ph.D.; Gonda, Koichi M.D.; Yoshimura, Kotaro M.D.

Institution Tokyo and Kanagawa, Japan
From the Department of Plastic Surgery, University of Tokyo School of Medicine, and the Division of Research and Development, Biomaster, Inc.

Title Influences of Preservation at Various Temperatures on Liposuction Aspirates.

Source Plastic & Reconstructive Surgery. 120(6):1510-1517, November 2007.

Abstract
Background: Aspirated fat is not only a filler material but also an abundant source of adipose-derived stem cells. The aim of this study was to assess degeneration of aspirated fat during preservation and optimize the preservation method for lipoaspirates.

Methods: Aspirated fat was preserved at room temperature for 1, 2, 4, and 24 hours (n = 10 each); at 4[degrees]C for 1, 2, and 3 days (n = 14 each); or at -80[degrees]C for 1 month (n = 3). Morphologic changes were assessed with scanning electron microscopy. Adipose-derived stem cell yield was measured after 1 week of culture. For aspirated fat preserved at room temperature, damaged adipocytes were assessed by measuring the oil volume ratio after centrifugation (n = 6) and glycerol-3-phosphate-dehydrogenase activity in washing solution (n = 4). Cell surface marker expression was examined by flow cytometry (n = 3).

Results: Although the scanning electron microscopic assay indicated no remarkable anatomical changes based on preservation methods, oil volume significantly increased in fat preserved at room temperature for 4 hours. Adipose-derived stem cell yield was significantly reduced by preservation at room temperature for 24 hours and by preservation at 4[degrees]C for 2 or 3 days. Flow cytometric analysis suggested that the biological properties of adipose-derived stem cells did not significantly change at 4[degrees]C up to 3 days. The cells were isolated from cryopreserved fat, but the yield was much less than that from fresh aspirated fat.

Conclusions: Aspirated fat should be transplanted as quickly as possible if it is preserved at room temperature. For adipose-derived stem cell isolation, aspirated fat can be stored or transported overnight if it is preserved at 4[degrees]C without adipose-derived stem cell yield loss or changes in biological properties.




這張圖是用電子顯微鏡去看脂肪細胞,結果形態沒有什麼變化,但實際上細胞已經壞死了

常常有診所宣稱可以把客人抽出來的脂肪保存,3~6個月內若覺得那邊補的不夠可以用保存的脂肪再注入即可。很多人被這種說法洗腦,會跟我說:你可以像xx診所一樣3個月內再補充一次嗎?我很懶得解釋,人性總喜歡偷懶及佔便宜,這種廣告手法便是源自人性的弱點。讓我們來看專家實驗結果:

作者把抽出來的脂肪分成三類:室溫保存,四度C保存(冰箱冷藏溫度),及-80度C保存。結果顯示在室溫下幾乎無法保存(最多4個小時),在四度C則可以放1天。至於在-80度C裡放30天後,能夠分離出來的幹細胞(adipose-derived stem cells)也遠比新鮮的脂肪少。

這個結論告訴我們即使那些宣稱有多高級設備的診所能夠將你抽出的脂肪保存在-80度C,隔了一個月後,這些脂肪還是遠比不上重新抽出的脂肪好。更何況有診所會買一台液態氮機器來儲存脂肪嗎?就我所知:沒有。至於那些放在冰箱的診所就別提了,頂多只能放一天。

_________________
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